The Argentina international landed heavily five minutes into Sunday’s match after hurdling goalkeeper Salvatore Sirigu when trying to reach Miralem Pjanic’s throughball.
The 29-year-old tried to continue after receiving treatment but eventually had to be replaced by Federico Bernardeschi. It appeared Higuain had hurt his ankle.
It will come as a concern for Juve boss Massimiliano Allegri ahead of a crucial run of fixtures in his side’s season.
Juve take on Atalanta in Serie A next Sunday before the second leg of the Coppa Italia semi-final against the same opponents three days later.
They then begin March with a trip to Lazio, four days before they take on Tottenham in the Champions League last 16, who hold the edge in the tie on away goals after a 2-2 draw in Turin.
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